March 1, 1992AJP Cell Physiology

Intracellular Ca2+ concentration and pHi during metabolic inhibition

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Population

Rat ventricular myocytes dual loaded with fura-2/AM and BCECF/AM

Comparison

Perfusion with 2 mM sodium cyanide for 30 min or… vs Control conditions

Design

Preclinical

Follow-up

30 min

Authors

HHHisayoshi HayashiUniversity of Shizuoka HMH. MiyataNational Institutes of Health NNNaohisa NodaHamamatsu University

Discussion

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Implication

Calcium dysregulation during metabolic inhibition may drive myocyte injury; leaves open mechanistic translation to human ischemia.

Structured PICO

Population

Rat ventricular myocytes dual loaded with fura-2/AM and BCECF/AM

Intervention

Perfusion with 2 mM sodium cyanide (NaCN) for 30 min (with or without 50 mM glucose) or acidic solution (70% O2-30% CO2)

Comparator

Control conditions

Outcome

Changes in intracellular Ca2+ concentration ([Ca2+]i), pH (pHi), and cell shape (percent of rod-shaped cells)surrogate

Metabolic inhibition in rat ventricular myocytes causes an increase in intracellular calcium and changes in cell shape, which are likely related to metabolic energy depletion and are not prevented by intracellular acidosis.

Cite This Study

Hayashi et al. (1992) studied this question.

synapsesocial.com/papers/6a8a42a09be9d02581bd9cd5 https://doi.org/10.1152/ajpcell.1992.262.3.c628

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Intracellular Ca2+ concentration and pHi during metabolic inhibition

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Intracellular Ca2+ concentration and pHi during metabolic inhibition

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider