The study successfully cloned and characterized a novel inward rectifier K+ channel (RBHIK1) from rabbit heart, demonstrating its functional properties and high expression in ventricular muscle.
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Advances rabbit ventricular K+ channel characterization; leaves open translation to human electrophysiology or arrhythmias.
We have isolated a cDNA coding for an inward rectifier K+ channel (RBHIK1) from rabbit heart. The cloned cDNA encodes a protein of 427 amino acids with two putative transmembrane segments. The primary structure of RBHIK1 is highly homologous to that of IRK1 which is an inward rectifier K+ channel recently cloned from mouse macrophage by expression cloning. When expressed in Xenopus oocytes, RBHIK1 current showed strong inward rectification and was inhibited by extracellular Ba2+ and Cs+. RNA blot analysis revealed the expression of RBHIK1 mRNA in various rabbit tissues, especially high level in the ventricular muscle.
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Ishii et al. (1994) studied this question.
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