What question did this study set out to answer?

To compare the conformation, stability, and dissociation kinetics of antithrombin-thrombin complexes formed in the presence versus the absence of heparin.

August 1, 1983Biochemical JournalOpen Access

Properties of antithrombin-thrombin complex formed in the presence and in the absence of heparin

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Authors

ÅDÅke DanielssonSwedish Defence Research Agency IBIngemar BjörkSwedish University of Agricultural Sciences

Discussion

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Implication

Biochemical analysis demonstrates equivalent dissociation kinetics and stability of antithrombin-thrombin complexes formed with or without heparin, indicating a catalytic role for heparin.

Key Points

To compare the conformation, stability, and dissociation kinetics of antithrombin-thrombin complexes formed in the presence versus the absence of heparin.
Purified antithrombin-thrombin complexes using DEAE-agarose ion-exchange chromatography and matrix-linked heparin chromatography to isolate monomeric and aggregated forms.
Quantified first-order dissociation rate constants through thrombin activity release assays and SDS-PAGE analysis of cleaved, modified antithrombin.
Monomeric complexes formed with or without heparin exhibited similar conformations, heparin affinities, and first-order dissociation rate constants ranging from 2.3 × 10⁻⁶ s⁻¹ to 3.4 × 10⁻⁶ s⁻¹.
The kinetically stable complex dissociated into active thrombin and cleaved, inactive antithrombin with a half-life of approximately 3 days, with no release of intact antithrombin detected.
Purification via matrix-linked heparin induced complex aggregation, which caused a marked decrease in the apparent dissociation rate.

Cite This Study

Danielsson et al. (1983) studied this question.

synapsesocial.com/papers/6a8a5e5d5c4c6b099dec923f https://doi.org/10.1042/bj2130345

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Implication

Biochemical analysis demonstrates equivalent dissociation kinetics and stability of antithrombin-thrombin complexes formed with or without heparin, indicating a catalytic role for heparin.

Properties of antithrombin-thrombin complex formed in the presence and in the absence of heparin

Discussion

Member takes

Implication

Key Points

Cite This Study

Properties of antithrombin-thrombin complex formed in the presence and in the absence of heparin

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider