July 15, 2005Molecular and Cellular BiologyOpen Access

Binding of hnRNP L to the Pre-mRNA Processing Enhancer of the Herpes Simplex Virus Thymidine Kinase Gene Enhances both Polyadenylation and Nucleocytoplasmic Export of Intronless mRNAs

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Population

CV-1 cells, cell-free polyadenylation system, and Xenopus oocytes

Comparison

2xTK49 pre-mRNA processing enhancer (PPE) element vs Intronless beta-globin RNA without the enhancer…

Design

Preclinical

Authors

SGShouhong GuangUniversity of Science and Technology of China AFAlicia FelthauserUniversity of Minnesota JMJanet E. MertzUniversity of Wisconsin–Madison

Discussion

Member takes

Implication

May enable improved mRNA therapeutics; leaves open validation in cardiovascular models.

Structured PICO

Population

CV-1 cells, cell-free polyadenylation system, and Xenopus oocytes

Intervention

2xTK49 pre-mRNA processing enhancer (PPE) element

Comparator

Intronless beta-globin RNA without the enhancer or with mutant PPEs

Outcome

Stability, polyadenylation, and cytoplasmic accumulation of transcriptssurrogate

hnRNP L enhances stability, polyadenylation, and nucleocytoplasmic export of intronless mRNAs by recruiting cofactors normally recruited to intron-containing RNAs.

Cite This Study

Guang et al. (2005) studied this question.

synapsesocial.com/papers/6a8a9e46f8670bac6e338550 https://doi.org/10.1128/mcb.25.15.6303-6313.2005

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Binding of hnRNP L to the Pre-mRNA Processing Enhancer of the Herpes Simplex Virus Thymidine Kinase Gene Enhances both Polyadenylation and Nucleocytoplasmic Export of Intronless mRNAs

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Binding of hnRNP L to the Pre-mRNA Processing Enhancer of the Herpes Simplex Virus Thymidine Kinase Gene Enhances both Polyadenylation and Nucleocytoplasmic Export of Intronless mRNAs

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider