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August 14, 2003The Journal of PhysiologyOpen Access

Role of cyclic nucleotide phosphodiesterase isoforms in cAMP compartmentation following 2-adrenergic stimulation of ICa,L in frog ventricular myocytes

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Population

Frog ventricular myocytes

Comparison

Isoprenaline applied locally, either alone or in… vs Isoprenaline alone

Design

Preclinical

Authors

JJJonas JurevičiusVSVytenis Arvydas SkeberdisRFRodolphe Fischmeister

Discussion

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Overview

PDE3/4 inhibition disrupts cAMP compartmentation in frog myocytes; leaves open isoform roles in mammalian cardiomyocytes and precludes clinical inference.

Structured PICO

P
Population
Frog ventricular myocytes
I
Intervention
Isoprenaline (1 nM to 10 μM) applied locally, either alone or in the presence of PDE inhibitors (IBMX 100 μM, milrinone 3 μM, Ro 20-1724 3 μM), okadaic acid (3 μM), or H89 (1 μM)
C
Comparator
Isoprenaline alone
O
Outcome
Gradient of local vs. distant cAMP concentration (α) determined by local and distant responses of L-type Ca(2+) current (I(Ca,L))surrogate

PDE3 and PDE4 are the primary phosphodiesterase isoforms responsible for cAMP compartmentation following beta-2-adrenergic stimulation in frog ventricular myocytes.

Cite This Study

Jurevičius et al. (2003) studied this question.

synapsesocial.com/papers/6a8aa6d451cd0cd12ac163d7https://doi.org/10.1113/jphysiol.2003.045211
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Also Consider

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  1. 1Local response of L‐type Ca<sup>2+</sup> current to nitric oxide in frog ventricular myocytes2001 · 68 citations
  2. 2Cyclic guanosine 3',5'‐monophosphate regulates the calcium current in single cells from frog ventricle.1987 · 200 citations
  3. 3Lack of detectable change in cyclic AMP during the cardiac inotropic response to isoproterenol immobilized on glass beads.1975 · 31 citations
  4. 4Both β<sub>2</sub>- and β<sub>1</sub>-Adrenergic Receptors Mediate Hastened Relaxation and Phosphorylation of Phospholamban and Troponin I in Ventricular Myocardium of Fallot Infants, Consistent With Selective Coupling of β<sub>2</sub>-Adrenergic Receptors to G<sub>s</sub>-Protein2000 · 69 citations
  5. 5Cardiac protein phosphorylation: functional and pathophysiological correlates1998 · 77 citations