Serum quality is a critical factor influencing TMEV viral entry in RAW264.7 macrophage cultures.
Serum batch effects must be controlled in macrophage viral assays; leaves open reproducibility of prior TMEV entry data.
Macrophages are the main targets of Theiler's murine encephalomyelitis virus (TMEV) during persistent demyelinating infection of mice. Replication of TMEV in macrophages was previously shown to depend on their activation state. Here, we show that the quality of the serum used for culture drastically influences viral entry in RAW264.7 macrophages.
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Shaw-Jackson et al. (1997) studied this question.
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