Slo1 Tail Domains, but Not the Ca2+ Bowl, Are Required for the β1 Subunit to Increase the Apparent Ca2+ Sensitivity of BK Channels

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Population

Large-conductance Ca- and voltage-activated K channels assembled from alpha subunits and auxiliary beta1…

Comparison

Replacement of Slo1 tail domain with Slo3 tail… vs Wild-type Slo1 channels or channels without…

Design

Preclinical

Authors

Discussion

Pinpoints Slo1 tail necessity for beta1 Ca2+ sensitization of BK channels; leaves open relevance to native cardiovascular tissues.

Population

Large-conductance Ca(2+)- and voltage-activated K(+) (BK) channels assembled from alpha subunits (Slo1) and auxiliary beta1 subunits

Intervention

Replacement of Slo1 tail domain with Slo3 tail domain, or disruption of the Ca(2+) bowl by mutations

Comparator

Wild-type Slo1 channels or channels without beta1 subunits

Outcome

Apparent Ca(2+) sensitivity and voltage sensitivity of the channelsurrogate

The increase in apparent Ca2+ sensitivity induced by the beta1 subunit in BK channels requires Slo1 tail domains but not the Ca2+ bowl.

Qian et al. (2002) studied this question.

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