What is the clinical evidence from this study?

Study design: Other. Intervention: BKIP-1 vs. bkip-1 loss-of-function mutation. Primary outcome: SLO-2 single-channel open probability and chloride sensitivity.

December 13, 2017Scientific ReportsOpen Access

BKIP-1, an auxiliary subunit critical to SLO-1 function, inhibits SLO-2 potassium channel in vivo

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Key result

BKIP-1 acts as an inhibitory auxiliary subunit of the C. elegans SLO-2 potassium channel, significantly reducing its chloride sensitivity, activation rate, and single-channel open probability.

Population

C. elegans worm strains

Comparison

Presence of BKIP-1 auxiliary subunit vs Worm strains without BKIP-1

Design

Preclinical

Authors

LNLonggang NiuUniversity of Connecticut PLPing LiuXihua University YSYuan ShuiUniversity of Connecticut

Discussion

Member takes

Implication

Provides no immediate clinical application; extends basic electrophysiology by demonstrating a single auxiliary subunit can exert.

Structured PICO

Population

C. elegans nematodes used for electrophysiological and bimolecular fluorescence complementation assays to study SLO-2 potassium channel function.

Intervention

Presence of BKIP-1 auxiliary subunit

Comparator

Worm strains without BKIP-1

Outcome

SLO-2 channel function (chloride sensitivity, activation rate, and single-channel open probability)surrogate

BKIP-1 is the first example of a single auxiliary subunit exerting opposite effects on evolutionarily related channels (SLO-1 and SLO-2) in the same cells.

Limitations

The modulatory effects of BKIP-1 on SLO-2 are relatively weak compared with those of many mammalian Slo1 regulatory proteins.
It remains unclear whether the regulation of two different Slo channels by a single auxiliary protein occurs in mammalian systems.

Cite This Study

Niu et al. (2017) studied this question. BKIP-1 vs. bkip-1 loss-of-function mutation was evaluated on SLO-2 single-channel open probability and chloride sensitivity. BKIP-1 acts as an inhibitory auxiliary subunit of the C. elegans SLO-2 potassium channel, significantly reducing its chloride sensitivity, activation rate, and single-channel open probability.

synapsesocial.com/papers/6a8c0db9f71b672e3df4aec6 https://doi.org/10.1038/s41598-017-18052-z

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BKIP-1, an auxiliary subunit critical to SLO-1 function, inhibits SLO-2 potassium channel in vivo

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

BKIP-1, an auxiliary subunit critical to SLO-1 function, inhibits SLO-2 potassium channel in vivo

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider