What question did this study set out to answer?

To identify and characterize novel proteins that physically interact with and modulate large-conductance calcium-activated potassium (BK) channels.

April 1, 1998Journal of NeuroscienceOpen Access

dSLo Interacting Protein 1, a Novel Protein That Interacts with Large-Conductance Calcium-Activated Potassium Channels

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Population

Drosophila, Xenopus oocytes, and transfected tissue culture cells

Comparison

Coexpression of dSLIP1 with dSlo or hSlo BK… vs Expression of BK channels alone

Design

Preclinical

Authors

XXXiaoming XiaHenan Provincial People's Hospital BHBirgit HirschbergMerck & Co., Inc., Rahway, NJ, USA (United States)SSSarah M. SmolikOregon Health & Science University

Discussion

Member takes

Implication

Provides a new molecular handle on BK channel expression; leaves open its relevance to native excitability or disease models.

Key Points

To identify and characterize novel proteins that physically interact with and modulate large-conductance calcium-activated potassium (BK) channels.
Screened Drosophila libraries using a modified yeast two-hybrid interaction trap to isolate BK channel-binding partners.
Assessed physical interactions between dSLIP1 and BK channels through in vitro binding assays and coimmunoprecipitation in transfected cells.
Measured channel function using electrophysiology in Xenopus oocytes and analyzed channel localization via single-channel recordings and immunostaining.
Identified dSLIP1, a novel protein with partial homology to the alpha1 syntrophin PDZ domain that binds Drosophila dSlo and human hSlo channels.
Coexpression of dSLIP1 with dSlo or hSlo in Xenopus oocytes selectively decreased macroscopic BK currents, which were restored by coexpressing the dSLIP1-interacting channel domain.
Single-channel recordings and immunostaining demonstrated that dSLIP1 decreases current amplitude primarily by reducing the density of BK channels at the plasma membrane.

Structured PICO

Population

Drosophila, Xenopus oocytes, and transfected tissue culture cells

Intervention

Coexpression of dSLIP1 with dSlo or hSlo BK channels

Comparator

Expression of BK channels alone

Outcome

BK channel currents and number of BK channels in the plasma membranesurrogate

Identification of dSLIP1 as a novel protein that interacts with BK channels and reduces their currents by decreasing channel expression in the plasma membrane.

Cite This Study

Xia et al. (1998) studied this question.

synapsesocial.com/papers/6a71504435aa2c282ce29ec3 https://doi.org/10.1523/jneurosci.18-07-02360.1998

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dSLo Interacting Protein 1, a Novel Protein That Interacts with Large-Conductance Calcium-Activated Potassium Channels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

dSLo Interacting Protein 1, a Novel Protein That Interacts with Large-Conductance Calcium-Activated Potassium Channels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider