Ca ²⁺ -binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca ²⁺ -dependent activation

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Structured PICO

Population

Drosophila slowpoke BK channel (Dslo-C280) overexpressed in Escherichia coli and HEK293 cells

Intervention

Mutation (D5N5) of Dslo-C280 (five consecutive Asp residues of the 'Ca-bowl' motif changed to Asn)

Comparator

Wild-type Dslo-C280, various control proteins, and known EF-hand Ca2+-binding proteins

Outcome

Ca2+-binding activity and Ca2+ sensitivity for activationsurrogate

The study demonstrates a functional correlation between Ca2+ binding to a specific region of the BK protein and Ca2+-dependent activation.

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Animal data link Ca2+ binding to BK activation; leaves open relevance to human cardiovascular ion channel function.

Bian et al. (2001) studied this question.

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