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January 1, 1991Journal of Cardiovascular Pharmacology

Induction of Endothelin Secretion by Angiotensin II

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Population

Cultured rat vascular smooth muscle cells

Comparison

Endothelin-1 (up to 10 M) and Angiotensin II vs Control conditions

Design

Preclinical

Authors

TSTimothy Scott‐BurdenTexas Medical CenterTRThérèse J. ResinkUniversity of Cape TownAHAlfred W.A. HahnUniversity Hospital of Basel

Discussion

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Implication

Ang II stimulates VSMC growth independently of ET-1 in this model; leaves open ET-1 contributions in other species or disease states.

Key Points

  • To determine whether endothelin-1 mediates the growth-stimulatory and extracellular matrix-inducing effects of angiotensin II in cultured rat vascular smooth muscle cells.
  • Cultured rat vascular smooth muscle cells in quiescent conditions or medium supplemented with 1.0% mitogen-depleted, plasma-derived serum.
  • Treated cultures with endothelin-1 (up to 10^-7 M) or angiotensin II to evaluate proliferation, DNA synthesis via [3H]thymidine incorporation, and extracellular matrix glycoconjugate synthesis.
  • Assessed gene expression of growth factors and thrombospondin in quiescent cells following endothelin-1 exposure.
  • Endothelin-1 at maximal doses (10^-7 M) failed to stimulate [3H]thymidine incorporation or extracellular matrix glycoconjugate synthesis in quiescent smooth muscle cells.
  • In cultures containing 1.0% plasma-derived serum, endothelin-1 did not stimulate cellular proliferation or DNA synthesis, whereas angiotensin II actively stimulated both.
  • Endothelin-1 stimulated the expression of growth factor and thrombospondin genes in quiescent smooth muscle cells despite having no effect on proliferation.

Structured PICO

P
Population
Cultured rat vascular smooth muscle cells
I
Intervention
Endothelin-1 (ET-1) (up to 10(-7) M) and Angiotensin II
C
Comparator
Control conditions (quiescent cultures or medium containing 1.0% plasma-derived serum)
O
Outcome
Cell growth/proliferation (incorporation of [3H]thymidine into DNA) and synthesis of extracellular matrix glycoconjugate materialsurrogate

Angiotensin II stimulates growth of vascular smooth muscle cells independently of endothelin-1 production.

Cite This Study

Scott‐Burden et al. (1991) studied this question.

synapsesocial.com/papers/6a8c0ee152e1a91077e98e78https://doi.org/10.1097/00005344-199100177-00025
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Endothelin-1 and angiotensin-II stimulate delayed mitogenesis in cultured rat aortic smooth muscle cells: evidence for common signaling mechanisms.1994 · 87 citations
  2. 2Effects and Interactions of Endothelin-1 and Angiotensin II on Matrix Protein Expression and Synthesis and Mesangial Cell Growth1996 · 157 citations
  3. 3Constitutive endothelin-1 overexpression promotes smooth muscle cell proliferation via an external autocrine loop1994 · 139 citations
  4. 4Angiotensin II-induced mitogenesis of spontaneously hypertensive rat-derived cultured smooth muscle cells is dependent on autocrine production of transforming growth factor-beta.1992 · 185 citations
  5. 5Angiotensin II Is Mitogenic for Cultured Rat Glomerular Endothelial Cells1996 · 81 citations