A double cyclodextrin host (3b) shows the largest association constant reported for a modified cyclodextrin (Kass.= 2 030 000 mol–1 dm3; with ethyl orange); the greater (×224) degree of association of this guest with (3b) as compared with β-cyclodextrin, demonstrates the full collaboration of the two binding sites.
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Fujita et al. (1984) studied this question.