September 1, 1996Journal of VirologyOpen Access

Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization

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Population

Rabbit reticulocyte lysates programmed with monocistronic RNAs containing 5' ends of GBV-A or GBV-C fused…

Design

Preclinical

Authors

JSJ. N. SimonsAbbott (United States)SDSuresh M. DesaiIndian Institute of Technology Goa DSDerk E. SchultzUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

No change to GBV clinical practice; leaves open IRES and core protein roles in human infection pending mammalian validation.

Structured PICO

Population

Rabbit reticulocyte lysates programmed with monocistronic RNAs containing 5' ends of GBV-A or GBV-C fused in-frame with the chloramphenicol acetyltransferase (CAT) open reading frame

Intervention

Translation studies including site-specific mutagenesis and N-terminal sequencing

Outcome

Mechanism and sites of polyprotein initiation in GBV-A and GBV-C

GBV-A and GBV-C are unique members of the Flaviviridae that utilize internal ribosome entry sites for translation initiation and lack core-like proteins at their N termini.

Cite This Study

Simons et al. (1996) studied this question.

synapsesocial.com/papers/6a8cff5bf64a4c3baf99116d https://doi.org/10.1128/jvi.70.9.6126-6135.1996

View Full Paper

Ask AI

Bookmark

Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider