What question did this study set out to answer?

To evaluate the time course of terminal complement complex C5b-9 deposition in ischemic myocardium and assess how reperfusion alters this timeline.

March 1, 1994European Heart Journal

Early accumulation of the terminal complement-complex in the ischaemic myocardium after reperfusion

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Why the study?

Does reperfusion after myocardial ischemia accelerate the accumulation of the terminal complement complex C5b-9 in the myocardium?

Population

40 rabbits undergoing experimental myocardial infarction via circumflex coronary artery occlusion

Comparison

Coronary artery occlusion for 0.5 to 6 hours… vs Coronary artery occlusion for 0.5 to 29 hours…

Design

Preclinical

Follow-up

Up to 29 hours

Authors

DMD MatheyGarrahan Hospital JSJoachim SchoferMedizinisches Versorgungszentrum Prof. Mathey, Prof. Schofer HSH. SchäferUniversity of Stuttgart

Discussion

Member takes

Implication

Reperfusion may accelerate complement-mediated myocardial injury; hypothesis-generating for targeted inhibition in human reperfusion injury.

Key Points

To evaluate the time course of terminal complement complex C5b-9 deposition in ischemic myocardium and assess how reperfusion alters this timeline.
Assigned 40 rabbits to either circumflex coronary artery occlusion without reperfusion (n=17, occlusion lasting 0.5 to 29 h) or occlusion followed by reperfusion (n=23, occlusion lasting 0.5 to 6 h).
Quantified transmural myocardial C5b-9 accumulation using immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA).
In the non-reperfused group, C5b-9 accumulation in ischemic myocardium occurred only after 5 to 6 h of coronary artery occlusion.
In the reperfused group, significant C5b-9 deposition was detected rapidly after just 30 min of myocardial ischemia.

Structured PICO

Does reperfusion after myocardial ischemia accelerate the accumulation of the terminal complement complex C5b-9 in the myocardium?

Population

40 rabbits undergoing experimental myocardial infarction via circumflex coronary artery occlusion

Intervention

Coronary artery occlusion for 0.5 to 6 hours followed by reperfusion (n=23)

Comparator

Coronary artery occlusion for 0.5 to 29 hours without subsequent reperfusion (n=17)

Outcome

Time course of C5b-9 (terminal complement complex) deposition in transmural myocardial biopsies determined by immunohistochemistry and ELISAsurrogate

Reperfusion rapidly accelerates the accumulation of the terminal complement complex C5b-9 in ischemic myocardium, suggesting a potential role in the pathogenesis of reperfusion injury.

Cite This Study

Mathey et al. (1994) studied this question.

synapsesocial.com/papers/6a8d293aa4bc1cf4cf7aa83f https://doi.org/10.1093/oxfordjournals.eurheartj.a060516

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Implication

Reperfusion may accelerate complement-mediated myocardial injury; hypothesis-generating for targeted inhibition in human reperfusion injury.

Early accumulation of the terminal complement-complex in the ischaemic myocardium after reperfusion

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Early accumulation of the terminal complement-complex in the ischaemic myocardium after reperfusion

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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