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October 24, 2014Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Loss of β-catenin in valvular interstitial cells leads to aberrant chondrogenic nodules and increased nuclear expression of Sox9 and chondrogenic extracellular matrix proteins.

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Population

Cultured chicken embryo aortic valves and mice with PeriostinCre-mediated tissue-restricted loss of…

Comparison

Loss of Wnt/β-catenin signaling vs Normal Wnt/β-catenin signaling

Design

Preclinical

Key result

Loss of β-catenin in valvular interstitial cells leads to aberrant chondrogenic nodules and increased nuclear expression of Sox9 and chondrogenic extracellular matrix proteins.

Authors

MFMing FangCAChristina M. AlfieriAHAlexia Hulin

Discussion

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Member takes

Overview

No immediate clinical implications for myxomatous valve disease; leaves open β-catenin-Sox9 axis as therapeutic target pending human validation.

Structured PICO

P
Population
Cultured chicken embryo aortic valves and mice with Periostin(Postn)Cre-mediated tissue-restricted loss of β-catenin (Ctnnb1) in valvular interstitial cells
I
Intervention
Loss of Wnt/β-catenin signaling (via genetic knockout in mice and in vitro models)
C
Comparator
Normal Wnt/β-catenin signaling (wild-type controls)
O
Outcome
Sox9 nuclear localization, proteoglycan expression, and chondrogenic differentiation (nodule formation and gene expression)surrogate

β-Catenin limits Sox9 nuclear localization and inhibits chondrogenic differentiation during valve development and in adult aortic valve homeostasis, providing mechanistic insights into myxomatous valve disease.

Cite This Study

Fang et al. (2014) studied Heart valve disease. Loss of β-catenin was evaluated on Sox9 nuclear localization and chondrogenic extracellular matrix gene expression. Loss of β-catenin in valvular interstitial cells leads to aberrant chondrogenic nodules and increased nuclear expression of Sox9 and chondrogenic extracellular matrix proteins.

synapsesocial.com/papers/6a8dcc030db1783955dcdbbchttps://doi.org/10.1161/atvbaha.114.304579
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