Comparison of metabolic and functional parameters using cardiac 18F-FDG-PET in early to mid-adulthood male and female mice

Figures

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  Fig. 1. Changes in LVMV and cardiac %IA/g in ageing mice. a Representative cardiac PET scan images for LVMV and %IA/g are illustrated. The upper panel shows the accumulation of 18F-FDG in the left ventricle in the coronal, the horizontal long axis and the vertical long axis. The lower panel of the left ventricle shows the VOI where the 30% least hottest voxels were excluded. b Shows the LVMV and cardiac %IA/g for male (in blue) and female mice (in red) at 10, 14 and 18 weeks of age. All groups n = 8. All data represent mean ± SD. * p = 0.05, ** p < 0.01, *** p < 0.001

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  Fig. 2. Assessment of clinically relevant cardiac function parameters by three-dimensional PET imaging. a shows different PET images' axes at various left ventricle levels during end-systole (left) and end-diastole (right). b illustrates three-dimensional reconstruction of the left ventricle in the ROA view. Upper picture: in end-systole and lower picture in end-diastole. c Comparison of male and female mice cardiac PET parameters at different stages of follow up. Data of male mice are illustrated in blue, and female mice are in red. All groups n = 7–8. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

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  Fig. 3. ECG analysis in both genders. a depicts the ECG tracking during the PET scan. Small black crosses in the QRS complex demonstrate the time of the trigger event. b ECG assessment of the heart rate in both genders during the PET scans. c Correlation of heart rate with cardiac LVMV and %IA/g, respectively. All groups n = 7–8 at different time points and cumulative group size in correlations n = 45. All data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.