Key result
The GeneXpert Enterovirus Assay demonstrated 100% sensitivity and 100% specificity for detecting enteroviral RNA in cerebrospinal fluid, compared to 65% sensitivity and 100% specificity for routine real-time one-step RT-PCR.
Why the study?
Does the GeneXpert Enterovirus Assay improve the detection of enteroviral RNA compared to real-time one step RT-PCR in the cerebrospinal fluid of patients with meningitis?
Observational (n=109)
No
Does the GeneXpert Enterovirus Assay improve the detection of enteroviral RNA compared to real-time one step RT-PCR in the cerebrospinal fluid of patients with meningitis?
Absolute Event Rate: 100% vs 65%
The GeneXpert Enterovirus Assay is a highly sensitive and specific method for detecting enteroviral RNA in cerebrospinal fluid, outperforming routine real-time one-step RT-PCR.
May support GeneXpert for enteroviral meningitis diagnosis; leaves open prospective validation before practice change.
BACKGROUND: Enteroviruses (EVs) are the leading cause of aseptic meningitis worldwide. Detection of enteroviral RNA in clinical specimens has been demonstrated to improve the management of patient care, especially that of neonates and young children. FINDINGS: To establish a sensitive and reliable assay for routine laboratory diagnosis, we compared the sensitivity and specificity of the GeneXpert Enterovirus Assay (GXEA) with that of the reverse transcription polymerase chain reaction (RT-PCR) based assay referred to as real-time one step RT-PCR (RTo-PCR). The sensitivity/specificity produced by GXEA and RTo-PCR were 100%/100% and 65%/100%, respectively. CONCLUSIONS: Both methods evaluated in this article can be used for detection of enterovirus in clinical specimens and these nucleic acid amplification methods are useful assays for the diagnosis of enteroviral infection.
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Hong et al. (2015) conducted an observational in Aseptic meningitis (n=109). GeneXpert Enterovirus Assay (GXEA) vs. Real-time one step RT-PCR (RTo-PCR) was evaluated on Sensitivity for the detection of enteroviral RNA in cerebrospinal fluid. The GeneXpert Enterovirus Assay demonstrated 100% sensitivity and 100% specificity for detecting enteroviral RNA in cerebrospinal fluid, compared to 65% sensitivity and 100% specificity for routine real-time one-step RT-PCR.
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