Molecular evaluation of enteroviruses in the pathogenesis of idiopathic dilated cardiomyopathy
Key result
Enterovirus RNA was detected in 10% of heart tissue samples from patients with dilated cardiomyopathy compared to 16% of control tissues, not supporting a major role for enterovirus in DCM.
Why the study?
Does the presence of enterovirus in heart tissue play a major role in the pathogenesis of idiopathic dilated cardiomyopathy?
Population
Patients with idiopathic dilated cardiomyopathy and control subjects providing biopsy or explant tissue.
Comparison
Molecular evaluation using reverse transcriptase… vs Control tissues.
Design
Case-control
Authors
Discussion
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Implication
Enterovirus presence does not differ in DCM versus controls; leaves open alternative etiologies and does not support targeted antiviral strategies.
Study Design
Type
Case-Control
Structured PICO
Does the presence of enterovirus in heart tissue play a major role in the pathogenesis of idiopathic dilated cardiomyopathy?
P
Population
Patients with dilated cardiomyopathy and control subjects whose heart tissue was tested for the presence of enterovirus.
E
Exposure
Molecular evaluation using reverse transcriptase polymerase chain reaction (PCR) and direct sequencing of RNA from heart tissue.
C
Comparator
Control tissues.
O
Outcome
Presence of enterovirus RNA and nucleotide sequence.surrogate
Main Result
Absolute Event Rate: 10% vs 16%
The study found no significant difference in enterovirus presence between DCM and control tissues, suggesting enterovirus does not play a major role in the pathogenesis of idiopathic dilated cardiomyopathy.
Limitations
- Studies using single biopsies produce consistently lower estimates for enterovirus than do those wherein multiple biopsies are examined.
- Use of single biopsies, which may produce consistently lower estimates for enterovirus detection compared to multiple biopsies.
Cite This Study
Kelling et al. (1997) conducted a case-control in Idiopathic dilated cardiomyopathy. Enterovirus vs. Controls was evaluated on Positive PCR signals for enterovirus. Enterovirus RNA was detected in 10% of heart tissue samples from patients with dilated cardiomyopathy compared to 16% of control tissues, not supporting a major role for enterovirus in DCM.