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October 1, 1993Cardiovascular Research

Changes in mitochondrial calcium concentration during the cardiac contraction cycle

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Population

Isolated guinea pig ventricular myocytes

Design

Preclinical

Authors

GIG IsenbergElectrophysiologyASA SchieferMayo ClinicMWM.F. Wendt-GallitelliUniversity of Cologne

Discussion

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Implication

Dynamic mitochondrial calcium cycling occurs in animal hearts; leaves open its role in human disease and requires translational validation.

Structured PICO

P
Population
Isolated guinea pig ventricular myocytes
I
Intervention
Paired voltage clamp pulses (2 mM [Ca2+]o, 36 degrees C) and block of mitochondrial Ca2+ uptake with DNP (100 microM) or FCCP (10 microM)
O
Outcome
Concentration of total calcium in mitochondria and surrounding cytosol during the contraction cyclesurrogate

This preclinical study demonstrates that mitochondrial calcium content changes dynamically during each individual cardiac contraction cycle, with substantial uptake during systole and release during later systole and diastole.

Limitations

  • Evaluation of mitochondrial Ca2+ uptake from DNP sensitive difference signals was hampered by sequestration of mitochondrial Ca2+ into the sarcoplasmic reticulum

Cite This Study

Isenberg et al. (1993) studied this question.

synapsesocial.com/papers/6a90424fe73a4c404fc92c8dhttps://doi.org/10.1093/cvr/27.10.1800
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Elevated Cytosolic Na <sup>+</sup> Decreases Mitochondrial Ca <sup>2+</sup> Uptake During Excitation-Contraction Coupling and Impairs Energetic Adaptation in Cardiac Myocytes2006 · 370 citations
  2. 2Rapid frequency‐dependent changes in free mitochondrial calcium concentration in rat cardiac myocytes2017 · 44 citations
  3. 3Measurement of mitochondrial free Ca2+ concentration in living single rat cardiac myocytes1991 · 277 citations
  4. 4Total and free myoplasmic calcium during a contraction cycle: x‐ray microanalysis in guinea‐pig ventricular myocytes.1991 · 95 citations
  5. 5Changes in mitochondrial function induced in isolated guinea‐pig ventricular myocytes by calcium overload.1994 · 59 citations