Key result
High-fat diet feeding for 24 weeks in mice resulted in reduced myocardial perfusion reserve compared to a control diet as measured by a novel MRI technique.
Accelerated dual-contrast first-pass MRI can successfully measure myocardial blood flow in free-breathing mice, detecting reduced myocardial perfusion reserve in diet-induced obesity.
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Should not yet alter clinical practice in obesity-related risk; leaves open translation of accelerated MRI perfusion imaging to human studies.
PURPOSE: Gene-modified mice may be used to elucidate molecular mechanisms underlying abnormal myocardial blow flow (MBF). We sought to develop a quantitative myocardial perfusion imaging technique for mice and to test the hypothesis that myocardial perfusion reserve (MPR) is reduced in a mouse model of diet-induced obesity (DIO). METHODS: A dual-contrast saturation-recovery sequence with ky -t undersampling and a motion-compensated compressed sensing reconstruction algorithm was developed for first-pass MRI on a small-bore 7 Tesla system. Control mice were imaged at rest and with the vasodilators ATL313 and Regadenoson (n = 6 each). In addition, we imaged mice fed a high-fat diet (HFD) for 24 weeks. RESULTS: In control mice, MBF was 5.7 ± 0.8 mL/g/min at rest and it increased to 11.8 ± 0.6 mL/g/min with ATL313 and to 10.4 ± 0.3 mL/g/min with Regadenoson. In HFD mice, we detected normal resting MBF (5.6 ± 0.4 versus 5.0 ± 0.3 on control diet), low MBF at stress (7.7 ± 0.4 versus 10.4 ± 0.3 on control diet, P < 0.05), and reduced MPR (1.4 ± 0.2 versus 2.0 ± 0.3 on control diet, P < 0.05). CONCLUSION: Accelerated dual-contrast first-pass MRI with motion-compensated compressed sensing provides spatiotemporal resolution suitable for measuring MBF in free-breathing mice, and detected reduced MPR in DIO mice. These techniques may be used to study molecular mechanisms that underlie abnormal myocardial perfusion.
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Naresh et al. (2014) studied Diet-induced obesity. High-fat diet vs. Control diet was evaluated on Myocardial perfusion reserve (MPR) (p=< 0.05). High-fat diet feeding for 24 weeks in mice resulted in reduced myocardial perfusion reserve compared to a control diet as measured by a novel MRI technique.
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