What question did this study set out to answer?

To characterise local myocardial overflow and tissue accumulation of endothelin during ischaemia and reperfusion, determine its coronary vascular effects, and examine modulation by L-arginine.

January 1, 1995Cardiovascular Research

Local overflow and enhanced tissue content of endothelin following myocardial ischaemia and reperfusion in the pig: modulation by L-arginine

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Why the study?

Does L-arginine modulate the local overflow and tissue content of endothelin following myocardial ischaemia and reperfusion in pigs?

Population

Anaesthetised pigs with induced myocardial ischaemia followed by 4 h of reperfusion

Comparison

Coronary venous retroinfusion of L-arginine… vs Non-ischaemic myocardial area; baseline conditions

Design

Preclinical

Follow-up

45 min ischaemia followed by 4 h reperfusion

Authors

QWQing‐Dong WangChina Animal Disease Control Center AHAnette HemsénKarolinska Institutet XLXing-San LiQiqihar Medical University

Discussion

Member takes

Implication

L-arginine attenuates endothelin rise in porcine ischemia-reperfusion; hypothesis-generating with no clinical implications yet.

Key Points

To characterise local myocardial overflow and tissue accumulation of endothelin during ischaemia and reperfusion, determine its coronary vascular effects, and examine modulation by L-arginine.
Anaesthetised pigs underwent 45 min of left anterior descending coronary artery occlusion followed by 4 h of reperfusion.
Endothelin-like immunoreactivity was measured in aortic and anterior interventricular coronary venous plasma and in left ventricular tissue, with a subgroup receiving coronary venous retroinfusion of L-arginine.
Local endothelin overflow significantly increased between 10 and 120 min of reperfusion, with tissue concentrations reaching 161 (SEM 30.5) fmol·g⁻¹ in ischaemic/reperfused myocardium compared to 25.3 (3.8) fmol·g⁻¹ in non-ischaemic myocardium (P < 0.05).
Coronary venous retroinfusion of L-arginine attenuated myocardial tissue endothelin-like immunoreactivity accumulation by 70% (P < 0.01) without altering plasma overflow.
Intracoronary endothelin-1, endothelin-3, and [Ala1,3,11,15]ET-1 induced dose-dependent coronary vasoconstriction and decreased left ventricular contractility, with endothelin-1 showing twofold greater potency than endothelin-3 and tenfold greater potency than [Ala1,3,11,15]ET-1.

Structured PICO

Does L-arginine modulate the local overflow and tissue content of endothelin following myocardial ischaemia and reperfusion in pigs?

Population

Anaesthetised pigs with induced myocardial ischaemia (45 min left anterior descending coronary artery ligation) followed by 4 h of reperfusion

Intervention

Coronary venous retroinfusion of L-arginine; intracoronary administration of endothelin analogues (endothelin-1, endothelin-3, and [Ala1,3,11,15]ET-1)

Comparator

Non-ischaemic myocardial area; baseline conditions

Outcome

Local myocardial overflow and tissue content of endothelin-like immunoreactivity (ET-LI)surrogate

Myocardial ischaemia and reperfusion evoke enhanced local tissue endothelin levels, which can be significantly attenuated by the nitric oxide substrate L-arginine.

Cite This Study

Wang et al. (1995) studied this question.

synapsesocial.com/papers/6a90e8b6d2f2d69b3a744f48 https://doi.org/10.1016/s0008-6363(96)88545-2

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Local overflow and enhanced tissue content of endothelin following myocardial ischaemia and reperfusion in the pig: modulation by L-arginine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Local overflow and enhanced tissue content of endothelin following myocardial ischaemia and reperfusion in the pig: modulation by L-arginine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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