Population
Rabbit myofibrils prepared from longissimus dorsi and leg muscles
Comparison
CASF treatment vs Trypsin treatment
Design
Preclinical
Authors
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CASF enables selective Z-disk studies with intact α-actinin; leaves open its role in postmortem or cardiac muscle proteolysis.
CASF specifically releases intact α-actinin from myofibril Z-disks, unlike trypsin which degrades it, suggesting a specific proteolytic mechanism relevant to postmortem muscle changes.
Suzuki et al. (1975) studied this question.
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