What question did this study set out to answer?

To determine the role of nitric oxide and downstream guanylate cyclase activation in histamine-induced macromolecular extravasation in postcapillary venules in vivo.

June 1, 1994AJP Heart and Circulatory Physiology

Nitric oxide accounts for histamine-induced increases in macromolecular extravasation

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WMWilliam G. MayhanUniversity of Nebraska at Omaha

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Implication

In vivo animal study demonstrates that nitric oxide mediates histamine-induced vascular leakage in hamsters, indicating a guanylate cyclase-dependent extravasation pathway.

Key Points

To determine the role of nitric oxide and downstream guanylate cyclase activation in histamine-induced macromolecular extravasation in postcapillary venules in vivo.
Examined macromolecular extravasation in postcapillary venules in hamster cheek pouches using intravital fluorescent microscopy and FITC-dextran (70 kDa).
Quantified venular leaky sites following superfusion with histamine (1.0 and 5.0 µM) in the presence or absence of L-NMMA, D-NMMA, L-arginine, or LY-83583.
Histamine increased venular leaky sites from zero under basal conditions to 11 ± 1 sites/0.11 cm² at 1.0 µM and 21 ± 2 sites/0.11 cm² at 5.0 µM.
Superfusion of the nitric oxide synthase inhibitor L-NMMA (1.0 µM) or the guanylate cyclase inhibitor LY-83583 (1.0 µM) significantly reduced histamine-induced leaky sites, while D-NMMA had no inhibitory effect.
Administration of L-arginine (100 µM) potentiated histamine-induced formation of venular leaky sites.

Cite This Study

William G. Mayhan (1994) studied this question.

synapsesocial.com/papers/6a915f91fbd14437d06f2f51 https://doi.org/10.1152/ajpheart.1994.266.6.h2369

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Implication

In vivo animal study demonstrates that nitric oxide mediates histamine-induced vascular leakage in hamsters, indicating a guanylate cyclase-dependent extravasation pathway.

Nitric oxide accounts for histamine-induced increases in macromolecular extravasation

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Implication

Key Points

Cite This Study

Nitric oxide accounts for histamine-induced increases in macromolecular extravasation

Discussion

Member takes

Implication

Key Points

Cite This Study

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