A horizontal, discontinuous gel electrophoresis system for protein separation is described. The method has been used for the analysis of proteins with markedly different properties (whey proteins, casein, milk clotting enzymes). The resolution was greater with this procedure than with the continuous acrylamide gel technique. The electrophoretic patterns were improved further by staining the gels with Coomassie Blue dye.
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Nicholas Melachouris (1969) studied this question.
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