Extremely efficient cleavage of eIF4G by picornaviral proteinases L and 2A in vitro
Population
Rabbit reticulocyte lysates in vitro model
Comparison
Translation of mRNAs encoding foot-and-mouth… vs Translation of mRNAs encoding human rhinovirus…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Lpro efficiency informs viral shutoff models; leaves open therapeutic targeting pending in vivo validation.
Structured PICO
P
Population
Rabbit reticulocyte lysates in vitro model
I
Intervention
Translation of mRNAs encoding foot-and-mouth disease virus leader proteinase (Lpro)
C
Comparator
Translation of mRNAs encoding human rhinovirus 2Apro
O
Outcome
Efficiency of eIF4G cleavage (time to 50% cleavage and molar ratios)surrogate
Lpro is significantly more efficient than 2Apro at cleaving eIF4G in vitro, with molar ratios similar to those observed during in vivo viral infection.
Cite This Study
Glaser et al. (2000) studied this question.