Protective outcomes of Zingiber officinale leaves ethanol extract on ovarian oxidative stress and hormonal functions in malaria mice

This study aimed to evaluate the impacts of ethanol extract of Zingiber officinale (EEZo) leaves on ovarian oxidative stress and hormonal functions in malaria mice. Mice were grouped into 6 groups of 6 mice each. After mice were euthanized, serum and ovarian tissues were taken for biochemical analyses. Parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and nitric oxide (NO) were assessed in the ovary. Estradiol, Anti-Mullerian Hormone (AMH), Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH) in the serum were estimated using the ELIAS Kit. The results shown significant low value of MDA (21.45 ± 0.71 μg/g wet tissue), high SOD (0.30 ± 0.14 μg/g wet tissue) and GSH (11.50 ± 1.27 μmol /g wet tissue) after treatment with EEZo at 400 mg/kg/d in comparison with malaria infected mice (60.0 ± 0.11 μg/g wet tissue; 0.05 ± 0.02 μg/g wet tissue; 1.50 ± 0.85 μmol /g wet tissue). Also, significant increase were observed in LH, FSH, AMH and estradiol in malaria infected group treated with EEZo and standard drug (Artemether and Lumefantrine (lonart). In conclusion, it is suggested that EEZo and Lonart could reduce oxidative stress and improve ovarian function in malaria mice.