What question did this study set out to answer?

To characterize antigenic determinants on feline leukemia virus (FeLV) envelope proteins using monoclonal antibodies and evaluate their neutralizing and cytotoxic capacities.

December 1, 1983The Journal of Immunology

Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.

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Authors

CGChris K. GrantInternational Medical Corps BEBarbara J. ErnisseHarvard University OJO. JarrettInstitut Pasteur

Discussion

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Implication

In vitro study reveals neutralizing and cytotoxic epitopes on envelope gp70 in feline leukemia virus producer cells, suggesting clustered functional targets for immunotherapy.

Key Points

To characterize antigenic determinants on feline leukemia virus (FeLV) envelope proteins using monoclonal antibodies and evaluate their neutralizing and cytotoxic capacities.
Generated and evaluated nine murine monoclonal antibodies directed against FeLV envelope proteins (eight against gp70 and one against p15E).
Tested antibodies for complement-dependent cytotoxicity against FeLV-producer lymphoma cells using cat and rabbit complement, and assayed virus-neutralizing activity across subgroups A, B, and C.
Mapped epitope spatial relationships across the gp70 molecule using competitive binding inhibition assays.
Four anti-gp70 monoclonal antibodies (two IgG2A and two IgG2B) were directly cytotoxic to FeLV-producer lymphoma cells, while a non-cytotoxic IgG2B antibody synergistically enhanced IgG2A-mediated cytotoxicity.
Cytotoxic antibodies demonstrated virus-neutralizing activity against subgroup A, B, and C determinants or determinants shared exclusively between subgroups B and C.
Competitive inhibition analysis mapped six discrete and two overlapping epitopes to a model shaped like an incomplete loop, with cytotoxic and neutralizing sites clustered near the center.

Cite This Study

Grant et al. (1983) studied this question.

synapsesocial.com/papers/6a93a57e9694293cf345338f https://doi.org/10.4049/jimmunol.131.6.3042

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Implication

In vitro study reveals neutralizing and cytotoxic epitopes on envelope gp70 in feline leukemia virus producer cells, suggesting clustered functional targets for immunotherapy.

Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.

Discussion

Member takes

Implication

Key Points

Cite This Study

Feline leukemia virus envelope gp70 of subgroups B and C defined by monoclonal antibodies with cytotoxic and neutralizing functions.

Discussion

Member takes

Implication

Key Points

Cite This Study

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