Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 28, 2007The Journal of PhysiologyOpen Access

Epac activation by 10 µM 8-CPT decreased [Ca2+]i transients (P<0.05) and increased Ca2+ spark frequency (from 2.4 to 6.9 sparks/s, P<0.01) while reducing their amplitude (P<0.001) in rat cardiomyocytes.

View Full Paper
Ask AI
Bookmark
Share

Population

Isolated adult rat cardiomyocytes

Comparison

Epac activation using the cAMP analogue… vs Control/baseline conditions without Epac…

Design

Preclinical

Key result

Epac activation by 10 µM 8-CPT decreased [Ca2+]i transients (P<0.05) and increased Ca2+ spark frequency (from 2.4 to 6.9 sparks/s, P<0.01) while reducing their amplitude (P<0.001) in rat cardiomyocytes.

Authors

LPLaëtitia PereiraMMMélanie MetrichMFMaría Fernández‐Velasco

Discussion

Loading...

Member takes

Overview

Epac-CaMKII Ca2+ effects in rat myocytes; leaves open relevance to human arrhythmias or HF.

Structured PICO

P
Population
Isolated adult rat cardiomyocytes used to analyze the effects of Epac activation on Ca2+ handling.
I
Intervention
Epac activation using the cAMP analogue 8-(4-chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate (8-CPT) (10 microm) and/or adenovirus encoding human Epac
C
Comparator
Control/baseline conditions without Epac activation
O
Outcome
Effects on Ca2+ handling including [Ca2+]i transients, Ca2+ sparks, L-type calcium current (ICa), and RyR phosphorylationsurrogate

Main Result

p-value: p=<0.05

Epac activation reveals a novel, PKA-independent cAMP signalling pathway that governs Ca2+ release and signalling in cardiac myocytes via CaMKII.

Cite This Study

Pereira et al. (2007) studied Isolated adult rat cardiomyocytes. Epac activation via 8-CPT vs. Baseline/Control was evaluated on [Ca2+]i transients and Ca2+ spark frequency (p=<0.05). Epac activation by 10 µM 8-CPT decreased [Ca2+]i transients (P<0.05) and increased Ca2+ spark frequency (from 2.4 to 6.9 sparks/s, P<0.01) while reducing their amplitude (P<0.001) in rat cardiomyocytes.

synapsesocial.com/papers/6a93cb561fcb3abe2907e1e5https://doi.org/10.1113/jphysiol.2007.133066
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The cAMP binding protein Epac regulates cardiac myofilament function2009 · 85 citations
  2. 2Depotentiation of intact rat cardiac muscle unmasks an Epac‐dependent increase in myofilament Ca<sup>2+</sup> sensitivity2015 · 12 citations
  3. 3Going to cAMP just got more complicated2007 · 27 citations
  4. 4Epac activation, altered calcium homeostasis and ventricular arrhythmogenesis in the murine heart2008 · 80 citations
  5. 5Exchange protein activated by cAMP (Epac) mediates cAMP‐dependent but protein kinase A‐insensitive modulation of vascular ATP‐sensitive potassium channels2009 · 67 citations