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August 7, 2009Proceedings of the National Academy of SciencesOpen Access

The cAMP binding protein Epac regulates cardiac myofilament function

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Population

Rat ventricular cardiac myocytes (intact and permeabilized)

Comparison

Selective Epac activation by the cAMP analog… vs Control cells infected with green fluorescent…

Design

Preclinical

Authors

OCOlivier CazorlaALAlexandre LucasFPFlorence Poirier

Discussion

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Overview

May modulate contractility via PKA-independent Epac signaling; hypothesis-generating for targeted therapies pending human studies.

Structured PICO

P
Population
Rat ventricular cardiac myocytes (intact and permeabilized)
I
Intervention
Selective Epac activation by the cAMP analog 8-(4-chlorophenylthio)-2'-O-methyl-cAMP (8-pCPT) or in vivo infection with a constitutively active form of Epac
C
Comparator
Control cells infected with green fluorescent protein (GFP) alone
O
Outcome
Myofilament Ca2+ sensitivity, cell shortening, Ca2+ transient amplitude, and phosphorylation of cardiac Troponin I (cTnI) and cardiac Myosin Binding Protein-C (cMyBP-C)surrogate

Epac is identified as a novel regulator of cardiac myofilament function, increasing Ca2+ sensitivity and phosphorylating key sarcomeric proteins independently of PKA.

Cite This Study

Cazorla et al. (2009) studied this question.

synapsesocial.com/papers/6a22d5cdc20dee76e681dbb8https://doi.org/10.1073/pnas.0812536106
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Depotentiation of intact rat cardiac muscle unmasks an Epac‐dependent increase in myofilament Ca<sup>2+</sup> sensitivity2015 · 13 citations
  2. 2The cAMP binding protein Epac modulates Ca 2+ sparks by a Ca 2+ /calmodulin kinase signalling pathway in rat cardiac myocytes2007 · 212 citations
  3. 3Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure2025
  4. 4cAMP-Binding Protein Epac Induces Cardiomyocyte Hypertrophy2005 · 181 citations
  5. 5Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure2024