Key result
PKA treatment and extracellular pH reduction independently and cumulatively increased the open probability of the cloned human ClC-2G(2 alpha) chloride channel expressed in oocytes.
Population
Human ClC-2G(2 alpha) Cl- channel cloned from a human fetal lung library and expressed in oocytes
Comparison
Adenosine 3',5'-cyclic monophosphate-dependent… vs Baseline conditions without PKA treatment and at…
Design
Preclinical
Authors
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Does not support clinical targeting of ClC-2; leaves open its role in human lung chloride transport pending in vivo studies.
The human ClC-2G(2 alpha) Cl- channel is activated by both PKA and low pH in a cumulative manner, suggesting a potential role in chloride transport in the human lung.
Sherry et al. (1997) studied this question. PKA treatment and pH reduction was evaluated on Channel probability of opening (Po). PKA treatment and extracellular pH reduction independently and cumulatively increased the open probability of the cloned human ClC-2G(2 alpha) chloride channel expressed in oocytes.
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