Key result
Partial bladder outlet obstruction in rats caused AT1 expression to disappear in the detrusor muscle, transiently increase in urothelial cells, and completely disappear by 14 days.
Why the study?
Does partial bladder outlet obstruction alter the expression of angiotensin II type 1 receptor in the rat bladder?
Does partial bladder outlet obstruction alter the expression of angiotensin II type 1 receptor in the rat bladder?
Partial bladder outlet obstruction in rats causes a transient shift of AT1 receptor expression to the urothelium before complete disappearance, suggesting a role in early bladder dysfunction.
Offers no immediate clinical implications for BOO; leaves open whether AT1 dynamics contribute to human bladder dysfunction.
PURPOSE: To demonstrate the change in the expression of angiotensin II type 1 receptor (AT1) in the rat bladder with partial bladder outlet obstruction (P-BOO). MATERIALS AND METHODS: Bladder specimens were obtained from 12-week-old Wistar female rats that were divided into two groups, a P-BOO group and a control group. The rats of the P-BOO group were divided into six groups: a sham-operated control group, 1 day postoperatively, 2 days postoperatively, 4 days postoperatively, 7 days postoperatively and 14 days postoperatively. The cystometric findings and immunohistochemical staining of the detrusor muscle with the AT1 antibody were compared in each group. RESULTS: AT1 localized on the cell membrane of the detrusor smooth muscle and in cytoplasm of suburothelial myofibroblasts in the control rats. The expression of AT1 disappeared in the detrusor muscle and suburothelial myofibroblasts in P-BOO, but AT1 was highly expressed in urothelial cells 1 day after surgery. The expression of AT1 in urothelial cells gradually decreased with time after surgery. AT1 completely disappeared in urothelial cells 14 days after surgery. CONCLUSIONS: The present study demonstrated that the site of AT1 expression changes in response to the mechanical stress caused by P-BOO, and finally there was no expression of AT1 in rat bladder tissue following P-BOO. These data suggest the change in AT1 expression may play a role in bladder function.
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Tobu et al. (2012) studied Partial bladder outlet obstruction. Partial bladder outlet obstruction (P-BOO) vs. Sham-operated control was evaluated on Expression of angiotensin II type 1 receptor (AT1) in the rat bladder. Partial bladder outlet obstruction in rats caused AT1 expression to disappear in the detrusor muscle, transiently increase in urothelial cells, and completely disappear by 14 days.
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