What question did this study set out to answer?

To determine the ultrastructural alterations that occur in cultured chick myocardial cells following exposure to electrical currents mimicking clinical countershock.

March 1, 1980Circulation ResearchOpen Access

Ultrastructural injury to chick myocardial cells in vitro following "electric countershock".

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Why the study?

Does electric field stimulation cause ultrastructural injury in cultured chick myocardial cells?

Population

Cultured chick myocardial cells (48 hours in culture)

Design

Preclinical

Follow-up

30 seconds postshock

Authors

JJJanice L. JonesUniversity of North Carolina at Chapel Hill CPCharmian C. ProskauerBoston University WPW. K. PaullUniversity of Vermont

Discussion

Member takes

Implication

May link shock intensity to post-defibrillation injury; leaves open translation from chick cells to clinical countershock protocols.

Key Points

To determine the ultrastructural alterations that occur in cultured chick myocardial cells following exposure to electrical currents mimicking clinical countershock.
Cultured chick myocardial cells were maintained in vitro for 48 hours and subjected to electric field stimulation at field strengths of 80 V/cm and 200 V/cm.
Cells were fixed 30 seconds following shock delivery and examined via electron microscopy to evaluate cellular and organelle morphology.
Exposure to 80 V/cm caused >25% of mitochondria per cell to assume a condensed configuration, alongside swirled or tubular cristae and focal swelling of the endoplasmic reticulum.
Exposure to 200 V/cm resulted in widespread myofibril contracture and disorganization, marked mitochondrial swelling with loss of cristae, swollen endoplasmic reticulum, dilated nuclear envelopes, and intracellular edema.
The severity of ultrastructural injury was directly dependent on shock intensity, consistent with an osmotic imbalance driven by membrane depolarization and transient dielectric breakdown.

Structured PICO

Does electric field stimulation cause ultrastructural injury in cultured chick myocardial cells?

Population

Cultured chick myocardial cells (48 hours in culture)

Intervention

Electric field stimulation (80 V/cm and 200 V/cm shocks)

Outcome

Ultrastructural alterations examined by electron microscope 30 seconds postshocksurrogate

High-intensity electric countershock causes direct, intensity-dependent ultrastructural damage to myocardial cells, which may underlie post-shock arrhythmias and necrosis.

Cite This Study

Jones et al. (1980) studied this question.

synapsesocial.com/papers/6a94ea50c163d75667fa5b31 https://doi.org/10.1161/01.res.46.3.387

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Ultrastructural injury to chick myocardial cells in vitro following "electric countershock".

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Ultrastructural injury to chick myocardial cells in vitro following "electric countershock".

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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