Key result
Decreasing pH from 7.2 to 5.5 increased the unfolding transition temperature of troponin C from 58°C to 67°C, suggesting stabilization of structural domains through carboxylate protonation.
Population
Rabbit fast skeletal muscle troponin C
Design
Preclinical
Authors
Loading...
No immediate clinical implications for troponin testing; leaves open pH effects on cardiac troponin C in vivo.
Troponin C consists of two types of structural domains that are stabilized through protonation of carboxylate groups in the Ca2+-binding loops.
Ingraham et al. (1983) studied this question. pH variation was evaluated on Unfolding transition temperature (tm). Decreasing pH from 7.2 to 5.5 increased the unfolding transition temperature of troponin C from 58°C to 67°C, suggesting stabilization of structural domains through carboxylate protonation.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: