What question did this study set out to answer?

To investigate the cellular mechanism by which angiotensin II potentiates ACTH-induced cAMP production in bovine adrenal glomerulosa cells.

February 15, 1998Biochemical JournalOpen Access

Angiotensin II potentiates adrenocorticotrophic hormone-induced cAMP formation in bovine adrenal glomerulosa cells through a capacitative calcium influx

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Population

Primary cultures of bovine adrenal glomerulosa cells

Comparison

Angiotensin II (100 nM) combined with ACTH vs ACTH alone, or Angiotensin II + ACTH in the…

Design

Preclinical

Authors

MBMuriel BurnayUniversity Hospital of Geneva BVB. Michel VALLOTTONUniversity Hospital of Geneva MCM. Alessandro CAPPONIUniversity Hospital of Geneva

Discussion

Member takes

Implication

Highlights calcium influx as modulator of adrenal cAMP; extends mechanistic insights but leaves open human relevance.

Key Points

To investigate the cellular mechanism by which angiotensin II potentiates ACTH-induced cAMP production in bovine adrenal glomerulosa cells.
Treated primary cultures of bovine adrenal glomerulosa cells with angiotensin II, ACTH, and channel modulators in the presence or absence of extracellular calcium (1.2 mM).
Pharmacologically modulated calcium entry using nicardipine, potassium ions, ionomycin, and thapsigargin to characterize the responsible calcium pathway.
Identified adenylyl cyclase isoform expression in bovine glomerulosa membranes via immunodetection.
Angiotensin II (100 nM) significantly potentiated maximal ACTH-induced cAMP formation in the presence of extracellular calcium (1.2 mM), but exerted no potentiating effect in calcium-free conditions or when administered alone.
Inhibition of voltage-operated calcium channels with nicardipine failed to block potentiation, whereas activation of capacitative calcium influx by thapsigargin mimicked the potentiating action of angiotensin II.
Immunodetection confirmed the presence of calcium-sensitive type III adenylyl cyclase in glomerulosa membranes, supporting its functional coupling to capacitative calcium entry.

Structured PICO

Population

Primary cultures of bovine adrenal glomerulosa cells

Intervention

Angiotensin II (100 nM) combined with ACTH

Comparator

ACTH alone, or Angiotensin II + ACTH in the absence of extracellular calcium

Outcome

cAMP formation/productionsurrogate

Angiotensin II potentiates ACTH-induced cAMP formation in bovine adrenal glomerulosa cells through a capacitative calcium influx likely coupled to type III adenylyl cyclase.

Cite This Study

Burnay et al. (1998) studied this question.

synapsesocial.com/papers/6a95d12d83a7209ed75396f5 https://doi.org/10.1042/bj3300021

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Angiotensin II potentiates adrenocorticotrophic hormone-induced cAMP formation in bovine adrenal glomerulosa cells through a capacitative calcium influx

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Angiotensin II potentiates adrenocorticotrophic hormone-induced cAMP formation in bovine adrenal glomerulosa cells through a capacitative calcium influx

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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