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December 1, 2016BMC AnesthesiologyOpen Access

Lidocaine relaxation in isolated rat aortic rings is enhanced by endothelial removal: possible role of Kv, KATP channels and A2a receptor crosstalk

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Key result

Lidocaine relaxation in rat thoracic aorta was biphasic and significantly enhanced by endothelial removal, an effect reduced by Kv, 5-HD, and A2a subtype inhibition.

Population

Male Sprague Dawley rats (300-350 g, n=72), from which thoracic aortic rings were harvested.

Comparison

Lidocaine applied to norepinephrine-precontracted… vs Intact vs denuded rings, and rings without…

Design

Preclinical

Authors

AAAryadi ArsyadGDGeoffrey P. Dobson

Discussion

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Overview

These animal data should not guide lidocaine use in patients; leaves open human endothelial-Kv channel interactions for study.

Structured PICO

P
Population
72 adult male Sprague Dawley rats provided isolated thoracic aortic rings to evaluate the vasoreactivity properties of lidocaine.
I
Intervention
Lidocaine (1 to 1000 μM) applied to norepinephrine-precontracted intact or denuded aortic rings, with or without inhibitors (L-NAME, indomethacin, 4-AP, glibenclamide, 5-HD, ouabain, CSC, PSB-0788).
C
Comparator
Intact vs denuded rings, and rings without inhibitors.
O
Outcome
Percentage relaxation of pre-contracted aortic rings (relative to maximal relaxation by 100 μM papaverine).surrogate

Lidocaine induces biphasic vasorelaxation in rat thoracic aorta that is enhanced by endothelial removal and mediated by Kv channels, MitoKATP channels, and A2a receptors.

Limitations

  • In vitro length-tension experiments without electrophysiological confirmation
  • Healthy rats used under normoxic and normal pH conditions
  • Other drug antagonists and agonists need to be examined

Cite This Study

Arsyad et al. (2016) studied Healthy (animal model) (n=72). Lidocaine vs. Intact endothelium and absence of inhibitors was evaluated on Percentage relaxation of norepinephrine pre-contracted aortic rings. Lidocaine relaxation in rat thoracic aorta was biphasic and significantly enhanced by endothelial removal, an effect reduced by Kv, 5-HD, and A2a subtype inhibition.

synapsesocial.com/papers/6a96369878d14db02cfa08dbhttps://doi.org/10.1186/s12871-016-0286-y
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Adenosine relaxation in isolated rat aortic rings and possible roles of smooth muscle Kv channels, KATP channels and A2a receptors2016 · 31 citations
  2. 2Contribution of nitric oxide and K+ channel activation to vasorelaxation of isolated rat aorta induced by procaine1999 · 26 citations
  3. 3Mechanisms Underlying the Endothelium-Independent Relaxation Induced by Angiotensin II in Rat Aorta2005 · 37 citations
  4. 4Mechanisms of calcium relaxation of vascular smooth muscle1991 · 26 citations
  5. 5Influence of the endothelium on Ca2+dependency of angiotensin II‐induced contractions of rat aortic rings1993 · 8 citations