February 1, 1971Journal of General Virology
Thermal Activation of the Antiviral Activity of Synthetic Polyribonucleotides: Influence of DEAE-dextran in Various Cell Cultures
Population
Four different cell lines (human skin fibroblasts, mouse embryo fibroblasts, mouse L 929 cells and rabbit…
Comparison
Alternating polyriboadenylic-polyribouridylic… vs Unheated poly r(A-U)
Design
Preclinical
Authors
Discussion
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Implication
Hypothesis-generating for thermally activated polynucleotides in antiviral therapy; leaves open in vivo efficacy and clinical translation.
Structured PICO
P
Population
Four different cell lines (human skin fibroblasts, mouse embryo fibroblasts, mouse L 929 cells and rabbit kidney (RK 13) cells)
I
Intervention
Alternating polyriboadenylic-polyribouridylic acid [poly r(A-U)] heated at 37° in minimal Eagle’s medium, with or without DEAE-dextran pre-treatment
C
Comparator
Unheated poly r(A-U)
O
Outcome
Cellular resistance to virus infection and interferon productionsurrogate
Heating poly r(A-U) enhances its antiviral and interferon-inducing activity in vitro, an effect modulated by DEAE-dextran.
Cite This Study
Clercq et al. (1971) studied this question.