Key result
Poly(2'-fluoro-2'-deoxyuridylic acid) inhibited the replication of vesicular stomatitis virus in mouse L cells by blocking the viral RNA-dependent RNA polymerase.
Absolute Event Rate: 9000000% vs 34000000%
Poly(2'-fluoro-2'-deoxyuridylic acid) inhibits vesicular stomatitis virus replication by blocking its RNA-dependent RNA polymerase, though its efficacy is limited by rapid degradation and poor cellular uptake.
Poly(2'-fluoro-2'-deoxyuridylic acid) is known to be an effective inhibitor of the deoxyribonucleic acid polymerase found within the oncornaviruses. This synthetic polynucleotide was found to inhibit the replication of vesicular stomatitis virus in mouse L cells. The polymer was shown to be capable of inhibiting the viral ribonucleic acid (RNA)-dependent RNA polymerase, and it is proposed that this is the mechanism of antiviral activity. The following observations support this viewpoint: (i) the polymer is most active when added after virus adsorption; (ii) the antiviral activity is not species specific; and (iii) the polynucleotide is nontoxic to the host cell. Conventional methodologies designed to increase nucleic acid uptake by cultured cells do not show an increase in antiviral potency.
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Grosch et al. (1975) studied Vesicular Stomatitis Virus infection (in vitro). Poly(2'-fluoro-2'-deoxyuridylic acid) [(dUfl)n] vs. Control (no polymer) was evaluated on Virus titer (PFU/ml) at 23 hours post-infection. Poly(2'-fluoro-2'-deoxyuridylic acid) inhibited the replication of vesicular stomatitis virus in mouse L cells by blocking the viral RNA-dependent RNA polymerase.
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