Key result
Insertion of the encephalomyocarditis virus (EMCV) IRES element between two open-reading frames of a plant bicistronic transcript can mediate translation of the second open-reading frame.
The EMCV IRES element can successfully mediate cap-independent translation of a second open-reading frame in plant cells, allowing for the functional use of bicistronic transcripts in plants.
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Enables bicistronic expression tools in plants; leaves open efficiency and species-specific validation for crop biotechnology.
Urwin et al. (2000) studied this question. Encephalomyocarditis virus (EMCV) IRES element was evaluated on Translation of the second open-reading frame in a bicistronic transcript. Insertion of the encephalomyocarditis virus (EMCV) IRES element between two open-reading frames of a plant bicistronic transcript can mediate translation of the second open-reading frame.
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