Key result
The newly engineered pPRIG vector, which retains the wild-type EMCV IRES initiation codon, yielded up to a 10-fold higher IRES-controlled protein expression compared to HindIII-modified vectors.
Population
Preclinical models using bicistronic expression vectors
Comparison
pPRIG vector family vs Existing bicistronic expression vectors with a…
Design
Preclinical
Authors
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Optimizes IRES-driven expression in animal models; extends vector tools but leaves open translation to cardiovascular gene therapy.
Absolute Event Rate: 234% vs 14%
The development of the pPRIG vector family restores optimal IRES function by preserving the authentic viral initiating AUG, overcoming the dramatic loss of expression seen in many existing commercial vectors.
Martin et al. (2006) studied this question. pPRIG vector (wild-type EMCV IRES) vs. HindIII-modified IRES vectors was evaluated on IRES-controlled expression (eGFP fluorescence peak value). The newly engineered pPRIG vector, which retains the wild-type EMCV IRES initiation codon, yielded up to a 10-fold higher IRES-controlled protein expression compared to HindIII-modified vectors.
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