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September 1, 2006BioTechniquesOpen Access

Translational Efficiency of EMCV IRES in Bicistronic Vectors is Dependent Upon IRES Sequence and Gene Location

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Population

Eukaryotic cells or cell-free extracts using dual-luciferase reporter constructs

Comparison

Native EMCV IRES in a bicistronic configuration vs pIRES vector and pCITE-1 monocistronic vector

Design

Preclinical

Authors

YBYury A. BochkovUniversity of Wisconsin–MadisonAPAnn C. PalmenbergUniversity of Wisconsin–Madison

Discussion

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Overview

Native EMCV IRES outperforms commercial variants in bicistronic expression; leaves open optimal vector selection for research use.

Structured PICO

P
Population
Eukaryotic cells or cell-free extracts using dual-luciferase reporter constructs
I
Intervention
Native EMCV IRES in a bicistronic configuration
C
Comparator
pIRES vector and pCITE-1 monocistronic vector
O
Outcome
Protein expression level (translational efficiency)surrogate

The translational efficiency of EMCV IRES vectors is highly dependent on the specific sequence used, with native sequences outperforming commercial variants like pIRES.

Cite This Study

Bochkov et al. (2006) studied this question.

synapsesocial.com/papers/6a215697baff55cd0402fd82https://doi.org/10.2144/000112243
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Also Consider

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  1. 1Development of a new bicistronic retroviral vector with strong IRES activity2006 · 56 citations
  2. 2Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs2001 · 187 citations
  3. 3Comparison of Picornaviral IRES-Driven Internal Initiation of Translation in Cultured Cells of Different Origins1997 · 236 citations
  4. 4Picornavirus internal ribosome entry segments: comparison of translation efficiency and the requirements for optimal internal initiation of translation<i>in vitro</i>1995 · 151 citations
  5. 5Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation1992 · 235 citations