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August 15, 2001Nucleic Acids ResearchOpen Access

Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs

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Key result

The composition of reading frames on a bicistronic mRNA and the order in which they are arranged define the strength of IRES-dependent translation.

Population

In vitro models using bicistronic expression vectors containing viral IRES elements, hepatitis B virus core…

Comparison

Varying the composition of reading frames and… vs Different cistron arrangements or monocistronic…

Design

Preclinical

Authors

MHMeike HenneckeMedizinische Hochschule Hannover

Discussion

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Overview

May guide bicistronic construct design in preclinical research; leaves open applicability to human therapeutics.

Structured PICO

P
Population
In vitro models using bicistronic expression vectors containing viral IRES elements (poliovirus and encephalomyocarditis virus), hepatitis B virus core antigen (HBcAg), cytokines, and reporter cistrons
I
Intervention
Varying the composition of reading frames and the order of cistron arrangement on bicistronic mRNAs
C
Comparator
Different cistron arrangements or monocistronic expression
O
Outcome
Strength of IRES-dependent translation of the second cistronsurrogate

The arrangement and composition of cistrons in bicistronic mRNAs are the dominant determinants of IRES-dependent translation efficiency.

Cite This Study

Meike Hennecke (2001) studied this question. Arrangement and composition of cistrons in bicistronic mRNAs was evaluated on Strength of IRES-dependent translation. The composition of reading frames on a bicistronic mRNA and the order in which they are arranged define the strength of IRES-dependent translation.

synapsesocial.com/papers/6a93c6001da36375f3d7c873https://doi.org/10.1093/nar/29.16.3327
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