What is the clinical evidence from this study?

Study design: Other. Population: Normal coronary arteries (porcine). Intervention: Female gender vs. Male gender. Primary outcome: Endothelium-dependent relaxations in coronary arteries.

What question did this study set out to answer?

To determine whether sex or normal fluctuations in endogenous sex steroid hormones alter endothelium-dependent coronary artery relaxation, and to identify the endothelium-derived factors responsible for any observed differences.

November 1, 1997AJP Heart and Circulatory Physiology

Gender differences in endothelium-dependent relaxations do not involve NO in porcine coronary arteries

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Key result

Endothelium-dependent relaxations to bradykinin and UK-14304 were significantly greater in coronary arteries from female compared with male pigs, involving indomethacin-sensitive cyclooxygenase products.

Population

Coronary arteries from sexually mature, gonadally intact male and female pigs or ovariectomized pigs

Comparison

Evaluation of endothelium-dependent relaxations… vs Male vs. female pigs; correlation with…

Design

Preclinical

Authors

DBDustan A. BarberMayo Clinic VMVirginia M. MillerCross-Cutting Cardiology

Discussion

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Implication

Porcine coronary findings should not alter clinical practice; leaves open whether cyclooxygenase products drive human sex differences in endothelial function.

Key Points

To determine whether sex or normal fluctuations in endogenous sex steroid hormones alter endothelium-dependent coronary artery relaxation, and to identify the endothelium-derived factors responsible for any observed differences.
Coronary arteries from sexually mature, intact male and female pigs as well as ovariectomized pigs were isolated to assess isometric force in organ chambers.
Vascular ring relaxations were evaluated in response to bradykinin and UK-14304, in the presence or absence of indomethacin and N(G)-monomethyl-L-arginine.
Vascular production of thromboxane A2 and prostacyclin, responsiveness to prostanoids, and calcium-dependent and calcium-independent nitric oxide synthase activities were quantified.
Arterial rings from female pigs showed significantly greater and leftward-shifted relaxations to bradykinin and UK-14304 compared with male rings, with no correlation to endogenous estrogen or progesterone levels.
Indomethacin (10^-5 mol/l) selectively increased endothelium-dependent relaxation in male coronary arteries, whereas nitric oxide synthase inhibition with N(G)-monomethyl-L-arginine reduced relaxation equally in both sexes.
Neither the baseline activity of calcium-dependent or calcium-independent nitric oxide synthase nor the production of and vascular response to thromboxane A2 and prostacyclin differed between sexes.

Structured PICO

Population

Sexually mature, gonadally intact male and female pigs or ovariectomized pigs used for in vitro study of coronary arteries.

Exposure

Evaluation of endothelium-dependent relaxations (using bradykinin, UK-14304, indomethacin, N(G)-monomethyl-L-arginine)

Comparator

Male vs. female pigs; correlation with endogenous sex steroid hormones

Outcome

Endothelium-dependent relaxations (isometric force), prostanoid production, and nitric oxide (NO) synthase activitysurrogate

Gender differences exist in endothelium-dependent relaxations of porcine coronary arteries, which are sensitive to indomethacin but independent of NO and normal fluctuations in endogenous sex steroid hormones.

Cite This Study

Barber et al. (1997) studied Normal coronary arteries (porcine). Female gender vs. Male gender was evaluated on Endothelium-dependent relaxations in coronary arteries. Endothelium-dependent relaxations to bradykinin and UK-14304 were significantly greater in coronary arteries from female compared with male pigs, involving indomethacin-sensitive cyclooxygenase products.

synapsesocial.com/papers/6a969bc4ff8a6b2115f6b6a8 https://doi.org/10.1152/ajpheart.1997.273.5.h2325

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Implication

Porcine coronary findings should not alter clinical practice; leaves open whether cyclooxygenase products drive human sex differences in endothelial function.

Gender differences in endothelium-dependent relaxations do not involve NO in porcine coronary arteries

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Gender differences in endothelium-dependent relaxations do not involve NO in porcine coronary arteries

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider