Key result
Dextran sulfate inhibited plaque formation of attenuated type 1 (Sabin) and type 2 (Lederle) polioviruses, but not virulent strains, providing a tool to discriminate wild from vaccine strains.
Dextran sulfate inhibits plaque formation of the attenuated type 1 Sabin poliovirus strain but not virulent strains, providing a potential in vitro tool to differentiate wild from vaccine strains.
May aid lab differentiation of poliovirus strains; leaves open validation for clinical or surveillance use.
Takemoto and Kirschstein (1) found that the attenuated strain of type 1 poliovirus (sabin) contained three different plaque types when assayed with agar overlay medium containing dextran sulfate, and further investigated several biological characteristics of the virus strains with the largest and the smallest plaque types. In the present study, the observations of Takemoto and Kirschstein are repeated on eleven virulent and attenuated strains of polioviruses and seven strains of Coxsackie viruses. Unexpectedly, the enhanced plaque formation as observed by them was not occurred in our experiments. The results, however, shows to be served as a possible tool that discriminate a wild virus strain from live poliovirus vaccine strain concering . at least, with type 1.
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Yamamoto et al. (1964) studied Enterovirus infection (in vitro). Dextran sulfate vs. No dextran sulfate was evaluated on Plaque size and number. Dextran sulfate inhibited plaque formation of attenuated type 1 (Sabin) and type 2 (Lederle) polioviruses, but not virulent strains, providing a tool to discriminate wild from vaccine strains.
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