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February 1, 2002The Journal of PhysiologyOpen Access

Comparison of the properties of CLCA1 generated currents and ICl(Ca) in murine portal vein smooth muscle cells

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Population

Freshly dispersed smooth muscle cells isolated from murine portal vein, murine cardiomyocytes, and HEK293…

Design

Preclinical

Authors

FBFiona C. BrittonUniversity of Nevada, Las VegasSOSusumu OhyaNagoya City UniversityBHBurton HorowitzUniversity of Nevada, Reno

Discussion

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Implication

Challenges mCLCA1 as the molecular basis of vascular I(Cl(Ca)); leaves open channel identity for further molecular studies.

Structured PICO

P
Population
Freshly dispersed smooth muscle cells isolated from murine portal vein, murine cardiomyocytes, and HEK293 cells stably transfected with mCLCA1 cDNA cloned from murine portal vein smooth muscle
I
Intervention
Electrophysiological characterization using whole-cell voltage clamp, RT-PCR, and exposure to Dithiothreitol (DTT, 1 mM)
O
Outcome
Comparison of electrophysiological properties (Ca2+ sensitivity, channel kinetics, modulation by DTT, and pore properties) between native I(Cl(Ca)) and mCLCA1 generated currentssurrogate

The study demonstrates that mCLCA1 alone does not account for the native calcium-activated chloride current in murine portal vein smooth muscle cells.

Cite This Study

Britton et al. (2002) studied this question.

synapsesocial.com/papers/6a970a6f132cd8a75e57ae45https://doi.org/10.1113/jphysiol.2001.013170
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular Characteristics And Functional Diversity Of Clca Family Members2000 · 104 citations
  2. 2Differential regulation of Ca2+‐activated Cl− currents in rabbit arterial and portal vein smooth muscle cells by Ca2+‐calmodulin‐dependent kinase2001 · 101 citations
  3. 3Modulation of the decay of Ca2+‐activated Cl− currents in rabbit portal vein smooth muscle cells by external anions1999 · 35 citations
  4. 4Bimodal Control of a Ca2+-Activated Cl− Channel by Different Ca2+ Signals1999 · 171 citations
  5. 5Cloning and Characterization of Lung-Endothelial Cell Adhesion Molecule-1 Suggest It Is an Endothelial Chloride Channel1997 · 95 citations