Key result
Exposure to 100 nmol/l ANP for 18 hours reduced ANP binding sites to 43% of control, but acid washing revealed no decrease in total receptor density.
Population
Cultured rat vascular smooth muscle cells (VSMC)
Comparison
Exposure to ANP, angiotensin II, arginine… vs Control (unexposed cells)
Design
Preclinical
Follow-up
18 h
Authors
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Apparent ANP receptor downregulation reflects occupancy rather than loss; leaves open whether this modulates vascular signaling in disease models.
In cultured rat vascular smooth muscle cells, ANP receptor density is not genuinely decreased by ANP, ANG II, AVP, or ET, although ANG II enhances ANP-stimulated cGMP production.
Schiffrin et al. (1991) studied Cultured rat vascular smooth muscle cells. ANP, angiotensin II, arginine vasopressin, and endothelin vs. Control was evaluated on Density of ANP binding sites. Exposure to 100 nmol/l ANP for 18 hours reduced ANP binding sites to 43% of control, but acid washing revealed no decrease in total receptor density.
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