Key result
Atrial natriuretic peptides exhibited reversible and saturable binding to A10 rat vascular smooth muscle cells with a Kd of 157 pM and Bmax of 115 fmol/mg of protein, stimulating intracellular cGMP.
Population
Cultured A10 rat vascular smooth muscle cell (VSMC) line
Design
Preclinical
Authors
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Establishes A10 cells as a model for ANP signaling; leaves open translation to human physiology and therapy.
The study demonstrates that A10 vascular smooth muscle cells possess specific ANP receptors coupled to cGMP stimulation, providing a useful model for studying ANP-induced vascular relaxation.
Neuser et al. (1986) studied this question. Atrial natriuretic peptides (ANP) was evaluated on Receptor binding characteristics (Kd, Bmax) and cGMP stimulation. Atrial natriuretic peptides exhibited reversible and saturable binding to A10 rat vascular smooth muscle cells with a Kd of 157 pM and Bmax of 115 fmol/mg of protein, stimulating intracellular cGMP.
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