Key result
Deletion of any segment of the extracellular domain of CAR resulted in loss of adhesion and mislocalization, while the D2 domain was required for adenovirus fiber-knob binding and infection.
Population
Preclinical models including CAR-positive monkey fibroblast cells, CAR-negative Chinese Hamster Ovary cells…
Comparison
Extracellular deletion mutants of the… vs Wild-type CAR (wt-CAR) or GFP control.
Design
Preclinical
Authors
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CAR domain mapping guides adenovirus vector design; leaves open human translation in respiratory or cardiac applications.
The entire extracellular domain of CAR, including the D2 domain, is vital for its junctional localization, cell adhesion, and ability to mediate adenovirus infection.
Excoffon et al. (2005) studied this question. Extracellular deletion mutants of CAR (CAR ΔD1, CAR ΔD2, CAR D0) vs. Wild-type CAR was evaluated on Cellular localization, adhesion, and viral infection. Deletion of any segment of the extracellular domain of CAR resulted in loss of adhesion and mislocalization, while the D2 domain was required for adenovirus fiber-knob binding and infection.
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