Key result
Partial sequencing of the VP1 region successfully typed 96% of enteroviral isolates, showing high agreement with conventional methods, whereas PCR-RFLP was inadequate for identification.
Answers are grounded in this paper and cite their sources.
Partial sequencing of the VP1 region is a rapid and accurate alternative to conventional LBM pool typing for enteroviral isolates, whereas PCR-RFLP is inadequate.
Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.
No takes yet. Share an insight, caveat, or question.
Manayani et al. (2002) studied Enterovirus infection (n=28). Partial sequencing of the VP1 region and PCR-RFLP vs. Lim-Benyesh-Melnick (LBM) pool-based neutralization was evaluated on Successful serotype assignment. Partial sequencing of the VP1 region successfully typed 96% of enteroviral isolates, showing high agreement with conventional methods, whereas PCR-RFLP was inadequate for identification.
Synapse has enriched 10 related papers. Consider them for comparative context: