A specific complement fixation test for the Lansing strain of poliomyelitis virus was successfully developed using CNS tissue from intraspinally infected mice, showing potential for serological diagnosis.
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Hypothesis-generating for serological diagnosis of Lansing poliomyelitis; prospective human validation required before clinical consideration.
Much higher spinal cord concentration of poliomyelitis virus can be obtained by using intraspinal inoculation technique of Habel and Li than by intracerebral technique. A complement fixing antigen was developed, using CNS tissue obtained from adult mice infected with this technique. This antigen produced a specific reaction with the sera of mice immunized with Lansing strain of poliomyelitis virus.The antigen and the immune serum of Lansing strain showed no cross reaction in complement fixation test against the antigens and the immune sera of Theiler's encephalomyelitis (TO strain). Japanese encephalitis (Nakayama strain), St. Louis encephalitis, Negishi strain, and lymphocytic choriomeningitis viruses. Furthermore, Lansing strain showed specific reaction in neutralization test with the immune serum of Lansing strain only, without reacting with the immune sera of any other neurotropic viruses.Two sera obtained from the cases suspected of nervous diseases fixed complement in the presence of Lansing strain antigen. Besides, their convalescent sera exhibited, following the elapses of time, an increase in the titer of neutralizing antibody against Lansing strain. As to whether or not the serological result and the clinical diagnosis for poliomyelitis coincide each other, or if coincide, to what grade, will be discussed with further investigations.
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