Key result
Exercise markedly induced ANGPTL4 expression in muscle, though liver and adipose tissue may contribute more to the exercise-induced increase in circulating ANGPTL4.
Why the study?
Does exercise regulate ANGPTL4 production in muscle and other tissues in humans and mice?
Does exercise regulate ANGPTL4 production in muscle and other tissues in humans and mice?
Exercise induces ANGPTL4 expression in muscle, fat, and liver, highlighting a multi-tissue response to regulate lipid metabolism during physical activity.
May inform exercise-lipid research in animals; leaves open human translation and clinical relevance.
Angiopoietin-like protein 4 (ANGPTL4) may regulate lipoprotein lipase-dependent plasma clearance of triacylglycerol from skeletal muscle during exercise. The aim of this study was to examine the importance of muscle in regulating ANGPTL4 in response to exercise. We sampled muscle biopsies and serum before, immediately after, and 2 h after 45 min of ergometer cycling. Sampling was done before and after a 12-week training intervention in controls and dysglycemic subjects. Moreover, fat biopsies were taken before and after the training intervention. The regulation of ANGPTL4 was also investigated in several tissues of exercising mice, and in cultured myotubes. ANGPTL4 levels in serum and expression in muscle were highest 2 h after exercise in both groups. Whereas ANGPTL4 was higher in muscle of exercising controls as compared to dysglycemic subjects, the opposite was observed in serum. In exercising mice, Angptl4 mRNA showed both higher basal expression and induction in liver compared to muscle. Angptl4 mRNA was much higher in adipose tissue than muscle and was also induced by exercise. We observed two mRNA isoforms of ANGPTL4 in muscle and fat in humans. Both were induced by exercise in muscle; one isoform was expressed 5- to 10-fold higher than the other. Studies in mice and cultured myotubes showed that both fatty acids and cortisol have the potential to increase ANGPTL4 expression in muscle during exercise. In conclusion, ANGPTL4 is markedly induced in muscle in response to exercise. However, liver and adipose tissue may contribute more than muscle to the exercise-induced increase in circulating ANGPTL4.
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Norheim et al. (2014) studied Dysglycemia. Exercise training intervention vs. Baseline (before exercise) and healthy controls was evaluated on ANGPTL4 levels in serum and expression in muscle. Exercise markedly induced ANGPTL4 expression in muscle, though liver and adipose tissue may contribute more to the exercise-induced increase in circulating ANGPTL4.
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